anti proliferating cell nuclear antigen pcna (Cell Signaling Technology Inc)
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anti proliferating cell nuclear antigen pcna
Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+proliferating+cell+nuclear+antigen+pcna/pm41715254-146-8-15
Average 86 stars, based on 1 article reviews
Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+proliferating+cell+nuclear+antigen+pcna/pm41715254-146-8-15
Average 86 stars, based on 1 article reviews
anti proliferating cell nuclear antigen pcna - by Bioz Stars,
2026-10
86/100 stars
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other:Article Title: The effects of low ambient temperature on steroidogenesis and mitochondrial functions in the testes of wild ground squirrels (Spermophilus dauricus). Article Snippet: Seasonal reproduction is a widely used breeding strategy in wildlife, especially vertebrates inhabiting temperate regions.. Generally, ambient temperature is considered a significant factor influencing the reproductive status of animals.. In the present study, wild ground squirrels (Spermophilus dauricus), typical seasonal breeders, were used as an animal model to investigate the mechanism behind the impact of low ambient temperature on testicular function. Article Title: Transferosome-mediated delivery of epigallocatechin three gallate and curcumin: An advanced therapeutic strategy for management of pancreatic cancer and lymphoma model Article Snippet: Background: Cancer remains a major global health issue, with lung cancer accounting for the highest mortality.. Pancreatic cancer, more prevalent among men, has a 5-year survival rate of less than 5 %.. Continued research into treatment options is crucial to combat pancreatic cancer. Staining:Article Title: Nur77 induced by HIF-1α mediates vascular remodeling in hypoxic pulmonary hypertension. Article Snippet: .. The paraffin-embedded lung section was stained with antiNur77 antibody (1:100; #sc-365113; Santa Cruz, Santa Cruz, CA, USA), anti-HIF-1α antibody (1:200; #ab2185; Abcam, Cambridge, MA, USA), Incubation:Article Title: The polyamine inhibitor SAM486A increases the efficacy of adagrasib in non-small cell lung cancer cells harboring KRAS G12C mutation. Article Snippet: Heat-Induced Epitope Retrieval (HIER) was performed for 20 minutes at 97°C using EnVision FLEX Target Retrieval Solution, High pH (Dako Omnis) in a PT-module from Thermo Scientific. .. Slides were incubated with a 1:4,000 dilution of Article Title: The polyamine inhibitor SAM486A increases the efficacy of adagrasib in non-small cell lung cancer cells harboring KRAS G12C mutation Article Snippet: Heat-Induced Epitope Retrieval (HIER) was performed for 20 minutes at 97 °C using EnVision FLEX Target Retrieval Solution, High pH (Dako Omnis) in a PT-module from Thermo Scientific. .. Slides were incubated with a 1:4,000 dilution of |
![hfNCSC-sEVs are taken up by PCs in vitro and enhance their proliferation and migration. (A) Primary cultures of hfNCSCs were established from male Sprague–Dawley rats. (B) Immunofluorescence staining of the neural crest cell marker p75 (red) and the stem cell marker nestin (green) in hfNCSCs, with 4′,6-diamidino-2-phenylindole (DAPI) staining indicating the nuclei. (C) Western blot analysis demonstrated the presence of surface markers (cluster of differentiation [CD]9, CD81, and tumor susceptibility gene 101 protein [TSG101]) and the absence of an endoplasmic reticulum marker (calnexin) in hfNCSC-sEVs. (D) Nanoparticle tracking analysis was used to quantify the concentration and size distribution of hfNCSC-sEVs. (E) Transmission electron microscopy was used to visualize the characteristic morphology of hfNCSC-sEVs. (F) Immunofluorescence staining indicated that the third-generation PCs cultured in vitro were positive for claudin-1, zonula occludens 1 (ZO1), and glucose transporter 1 (GLUT1) but negative for S100, with DAPI staining marking the nuclei. (G) The internalization of PKH26-labeled hfNCSC-sEVs (red) by ZO1-positive PCs (green) was visualized using immunofluorescence staining, with DAPI staining to mark the nuclei. (H) The Cell Counting Kit-8 assay was used to evaluate the cell viability of PCs across concentrations of 0, 2 × 10 8 , 5 × 10 8 , and 10 × 10 8 particles/mL hfNCSC-sEVs at 3, 5, and 7 days of in vitro culture ( n = 5 per group). (I) The Transwell assay was used to quantify the number of migrating PCs at 6, 12, and 18 hours post-treatment with the aforementioned concentrations of hfNCSC-sEVs, in in vitro culture ( n = 6 per group). (J) Western blot and (K) statistical analyses revealed the relative protein expression levels of proliferating cell nuclear antigen <t>(PCNA)</t> and vimentin in PCs from the phosphate-buffered saline (PBS) and hfNCSC-sEVs groups on day 5 of in vitro culture (normalized to β-actin, n = 3 per group). Data are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance and Tukey’s multiple comparison test for H and I; Student’s t -test for K). The data were from at least three separate and independent studies. CCK-8: Cell counting kit-8; GLUT1: glucose transporter 1; hfNCSCs: hair follicle neural crest stem cells; ns: not significant; PCNA: proliferating cell nuclear antigen; PCs: perineurial cells; sEVs: small extracellular vesicles; ZO1: zonula occludens 1.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4726/pmc12694726/pmc12694726__NRR-21-2060-g002.jpg)
![( A ) Schematic illustration of cytoplasmic relocalization of p65 mediated by the anchoring aptamer. ( B ) Schematic diagram of the p65 ligand (P028F4, an aptamer targeting p65), the Ras ligand (Ra1, an aptamer targeting Ras) and anchoring aptamers [PAR (P028F4-AAA-Ra1), RAP (Ra1-AAA-P028F4), PTR (P028F4-TTT-Ra1), and RTP (Ra1-TTT-P028F4)]. ( C ) Native polyacrylamide gel analysis of aptamers. Lane 1, single-stranded DNA (ssDNA) marker; lane 2, P028F4; lane 3, Ra1; lane 4, PAR; lane 5, RAP; lane 6, PTR; lane 7, RTP. nt, nucleotides. ( D ) H1299 cells were transfected with control library, PAR, RAP, PTR, or RTP (all at 200 nM) for 12 hours, followed by tumor necrosis factor–α (TNFα) stimulation (10 ng/ml for 1.5 hours). Cytoplasmic (cyto) and nuclear (nuc) p65 levels were analyzed by cellular fractionation and immunoblotting. Error bars represent means ± SD from three independent experiments ( n = 3). P values are indicated by Student’s t test. ( E ) H1299 cells were transfected with 200 nM RTP constructs containing linkers of different lengths for 12 hours, followed by TNFα stimulation (10 ng/ml for 1.5 hours). Cytoplasmic and nuclear p65 levels were analyzed by cellular fractionation and immunoblotting. ( F ) H1299 cells were transfected with RTP at varying concentrations (0, 100, 200, and 300 nM) for 12 hours, followed by TNFα stimulation (10 ng/ml for 1.5 hours). Cytoplasmic and nuclear p65 levels were assessed by cellular fractionation and immunoblotting. <t>Proliferating</t> cell nuclear antigen <t>(PCNA)</t> or H3 serves as the nuclear marker, and β-actin or vinculin serves as the cytoplasmic marker.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7074/pmc12757074/pmc12757074__sciadv.adw6496-f2.jpg)
